Clinical-scale laser-based scanning and processing of live cells: Selective photo-thermal killing of fluorescent tumor targets for autologous stem cell transplantation

Manfred R. Koller, Elie G. Hanania, Timothy Eisfeld, Robert A. O'Neal, Kevin M. Khovananth, Bernhard O. Palsson

Research output: Contribution to journalArticlepeer-review

Abstract

High-dose chemotherapy, followed by autologous hematopoietic stem cell (HSC) transplantation, is widely used for the treatment of cancer. However, contaminating tumor cells within HSC harvests continue to be of major concern since reinfused tumor cells have proven to contribute to disease relapse. Many tumor purging methods have been evaluated, but all leave detectable tumor cells in the transplant and result in significant loss of HSCs. These shortcomings cause engraftment delays and compromise the therapeutic value of purging. A novel approach integrating automated scanning cytometry, image analysis, and selective laser-induced killing of labeled cells within a cell mixture is described here. Non-Hodgkin's lymphoma (NHL) cells were spiked into cell mixtures, and fluorochrome-conjugated antibodies were used to label tumor cells within the mixture. Cells were then allowed to settle on a surface, and as the surface was scanned with a fluorescence excitation source, a laser pulse was fired ar every detected tumor cell using high-speed beam steering mirrors. Tumor cells were selectively killed with little effect on adjacent non-target cells, demonstrating the feasibility of this automated cell processing approach. This technology has many potential research and clinical applications, one example of which is tumor cell purging for autologous HSC transplantation.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4248
DOIs
Publication statusPublished - 2001

Other keywords

  • Cancer
  • Fluorescent scanning cytometry
  • Image analysis
  • Lasers
  • Photo-thermal effects
  • Stem cell transplantation
  • Tumor purging

Fingerprint

Dive into the research topics of 'Clinical-scale laser-based scanning and processing of live cells: Selective photo-thermal killing of fluorescent tumor targets for autologous stem cell transplantation'. Together they form a unique fingerprint.

Cite this