Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr (Salvelinus alpinus)

Dean Sibthorpe, Rán Sturlaugsdóttir, Bjarni K. Kristjansson, Helgi Thorarensen, Skúli Skúlason, Ian A. Johnston*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Arctic charr (Salvelinus alpinus L.) from Lake Thingvallavatn, Iceland occur as four distinct morphs: large benthivorous (LB), dwarf benthivorous (DB), piscivorous (PI) and planktonivorous (PL). The morphs differ with respect to body size, head morphology, growth rate, and life history. The aim of this study was to investigate the paired box protein 7 (Pax7) gene as a candidate for such polymorphisms due to its importance in cranio-facial, skeletal muscle, and central nervous system development. No variation in coding and intronic sequences was found between morphs. We identified 10 alternate Pax7 isoforms with insertions/deletions: a four-residue (GNRT) deletion, a GEASS insertion truncated by the first serine residue (GEAS), and a thirteen-residue insertion (GQYA/TGPEYVYCGT). The latter insertion with a threonine (T) contains a putative casein kinase II (CK-2) phosphorylation site. Pax7 spatial expression patterns were identical in embryos of DB-, LB-, and PL-morphs, and were similar to those described for zebrafish Pax7c, but a difference in temporal expression for segmentation was observed between DB and LB morphs. At the end of segmentation, novel expression was observed in the mandibular region as two bilateral domains. The potential role of multiple alternative splicing of the Pax7 gene for the generation of different Arctic charr morphs is briefly discussed.

Original languageEnglish
Pages (from-to)371-383
Number of pages13
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume145
Issue number3-4
DOIs
Publication statusPublished - 2006

Bibliographical note

Funding Information:
We are grateful to the Natural Environmental Research Council of the UK for funding the project (Grant NER/A/S/2000/00558) and to Susan Gotensparre for the generous donation of degenerate primers, and Sean Earnshaw for help with preparing the illustrations.

Other keywords

  • Arctic charr
  • Development
  • Evolution
  • Morph
  • Multiple splice variants
  • Pax7
  • Resource polymorphism
  • Salmonid
  • Salvelinus alpinus
  • Skeletal muscle

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