Authentication of Iceland Moss (Cetraria islandica) by UPLC-QToF-MS metabolite profiling and DNA barcoding.

Elín Soffía Ólafsdóttir; M. Xu; S. Heidmarsson; M. Thorsteinsdottir; M. Kreuzer; J. Hawkins; S. Omarsdottir

doi:10.1016/j.foodchem.2017.11.073

Authentication of Iceland Moss (Cetraria islandica) by UPLC-QToF-MS metabolite profiling and DNA barcoding.

Elín Soffía Ólafsdóttir, M. Xu , S. Heidmarsson , M. Thorsteinsdottir , M. Kreuzer , J. Hawkins , S. Omarsdottir

Research output: Contribution to journal › Article › peer-review

Abstract

The lichen Cetraria islandica or Iceland Moss is commonly consumed as tea, food ingredients (e.g. in soup or bread) and herbal medicines. C. islandica, which has two chemotypes, can be difficult to distinguish from the sister species Cetraria ericetorum. They are collectively referred to as the Cetraria islandica species complex. This study aimed to use an UPLC-QToF-MS chemical profiling together with DNA barcoding to distinguish species and chemotypes of the C. islandica species complex. Our results show that the two chemotypes of C. islandica are clearly distinguishable from each other and from C. ericetorum by the chemometric approach. The RPB2 barcode was able to differentiate C. islandica from C. ericetorum with a barcode gap, but the widely used nrITS barcode failed. Neither of them could discriminate chemotypes of C. islandica. In conclusion, this integrative approach involving chemical profiling and DNA barcoding could be applied for authentication of Iceland Moss materials.

Original language	English
Pages (from-to)	989-996
Number of pages	8
Journal	Food Chemistry
Volume	245
DOIs	https://doi.org/10.1016/j.foodchem.2017.11.073
Publication status	Published - 15 Apr 2018

Bibliographical note

Funding Information:
This work was supported by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme FP7/2007-2013/[Grant number 606895]; Bergthora and Thorsteinn Scheving Thorsteinsson Fund. Professor Kristinn P. Magnusson and Ph.D student Maney Sveinsdottir, University of Akureyri, Iceland, are acknowledged for their help in molecular analysis. The authors are particularly grateful to Dr. Hörður Kristinsson (AMNH) for specimen collection and thoughtful discussion.

Funding Information:
This work was supported by the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme FP7/2007-2013/[Grant number 606895]; Bergthora and Thorsteinn Scheving Thorsteinsson Fund. Professor Kristinn P. Magnusson and Ph.D student Maney Sveinsdottir, University of Akureyri, Iceland, are acknowledged for their help in molecular analysis. The authors are particularly grateful to Dr. H?r?ur Kristinsson (AMNH) for specimen collection and thoughtful discussion.

Publisher Copyright:
© 2017 Elsevier Ltd

Other keywords

Authentication
Cetraria ericetorum
Cetraria islandica
Chemical profiling
DNA barcoding

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10.1016/j.foodchem.2017.11.073

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title = "Authentication of Iceland Moss (Cetraria islandica) by UPLC-QToF-MS metabolite profiling and DNA barcoding.",

abstract = "The lichen Cetraria islandica or Iceland Moss is commonly consumed as tea, food ingredients (e.g. in soup or bread) and herbal medicines. C. islandica, which has two chemotypes, can be difficult to distinguish from the sister species Cetraria ericetorum. They are collectively referred to as the Cetraria islandica species complex. This study aimed to use an UPLC-QToF-MS chemical profiling together with DNA barcoding to distinguish species and chemotypes of the C. islandica species complex. Our results show that the two chemotypes of C. islandica are clearly distinguishable from each other and from C. ericetorum by the chemometric approach. The RPB2 barcode was able to differentiate C. islandica from C. ericetorum with a barcode gap, but the widely used nrITS barcode failed. Neither of them could discriminate chemotypes of C. islandica. In conclusion, this integrative approach involving chemical profiling and DNA barcoding could be applied for authentication of Iceland Moss materials.",

keywords = "Authentication, Cetraria ericetorum, Cetraria islandica, Chemical profiling, DNA barcoding",

author = "{\'O}lafsd{\'o}ttir, {El{\'i}n Soff{\'i}a} and M. Xu and S. Heidmarsson and M. Thorsteinsdottir and M. Kreuzer and J. Hawkins and S. Omarsdottir",

note = "Funding Information: This work was supported by the People Programme (Marie Curie Actions) of the European Union{\textquoteright}s Seventh Framework Programme FP7/2007-2013/[Grant number 606895]; Bergthora and Thorsteinn Scheving Thorsteinsson Fund. Professor Kristinn P. Magnusson and Ph.D student Maney Sveinsdottir, University of Akureyri, Iceland, are acknowledged for their help in molecular analysis. The authors are particularly grateful to Dr. H{\"o}r{\dh}ur Kristinsson (AMNH) for specimen collection and thoughtful discussion. Funding Information: This work was supported by the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme FP7/2007-2013/[Grant number 606895]; Bergthora and Thorsteinn Scheving Thorsteinsson Fund. Professor Kristinn P. Magnusson and Ph.D student Maney Sveinsdottir, University of Akureyri, Iceland, are acknowledged for their help in molecular analysis. The authors are particularly grateful to Dr. H?r?ur Kristinsson (AMNH) for specimen collection and thoughtful discussion. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2018",

month = apr,

day = "15",

doi = "10.1016/j.foodchem.2017.11.073",

language = "English",

volume = "245",

pages = "989--996",

journal = "Food Chemistry",

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AU - Ólafsdóttir, Elín Soffía

AU - Xu , M.

AU - Heidmarsson , S.

AU - Thorsteinsdottir , M.

AU - Kreuzer , M.

AU - Hawkins , J.

AU - Omarsdottir , S.

N1 - Funding Information: This work was supported by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme FP7/2007-2013/[Grant number 606895]; Bergthora and Thorsteinn Scheving Thorsteinsson Fund. Professor Kristinn P. Magnusson and Ph.D student Maney Sveinsdottir, University of Akureyri, Iceland, are acknowledged for their help in molecular analysis. The authors are particularly grateful to Dr. Hörður Kristinsson (AMNH) for specimen collection and thoughtful discussion. Funding Information: This work was supported by the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme FP7/2007-2013/[Grant number 606895]; Bergthora and Thorsteinn Scheving Thorsteinsson Fund. Professor Kristinn P. Magnusson and Ph.D student Maney Sveinsdottir, University of Akureyri, Iceland, are acknowledged for their help in molecular analysis. The authors are particularly grateful to Dr. H?r?ur Kristinsson (AMNH) for specimen collection and thoughtful discussion. Publisher Copyright: © 2017 Elsevier Ltd

PY - 2018/4/15

Y1 - 2018/4/15

N2 - The lichen Cetraria islandica or Iceland Moss is commonly consumed as tea, food ingredients (e.g. in soup or bread) and herbal medicines. C. islandica, which has two chemotypes, can be difficult to distinguish from the sister species Cetraria ericetorum. They are collectively referred to as the Cetraria islandica species complex. This study aimed to use an UPLC-QToF-MS chemical profiling together with DNA barcoding to distinguish species and chemotypes of the C. islandica species complex. Our results show that the two chemotypes of C. islandica are clearly distinguishable from each other and from C. ericetorum by the chemometric approach. The RPB2 barcode was able to differentiate C. islandica from C. ericetorum with a barcode gap, but the widely used nrITS barcode failed. Neither of them could discriminate chemotypes of C. islandica. In conclusion, this integrative approach involving chemical profiling and DNA barcoding could be applied for authentication of Iceland Moss materials.

AB - The lichen Cetraria islandica or Iceland Moss is commonly consumed as tea, food ingredients (e.g. in soup or bread) and herbal medicines. C. islandica, which has two chemotypes, can be difficult to distinguish from the sister species Cetraria ericetorum. They are collectively referred to as the Cetraria islandica species complex. This study aimed to use an UPLC-QToF-MS chemical profiling together with DNA barcoding to distinguish species and chemotypes of the C. islandica species complex. Our results show that the two chemotypes of C. islandica are clearly distinguishable from each other and from C. ericetorum by the chemometric approach. The RPB2 barcode was able to differentiate C. islandica from C. ericetorum with a barcode gap, but the widely used nrITS barcode failed. Neither of them could discriminate chemotypes of C. islandica. In conclusion, this integrative approach involving chemical profiling and DNA barcoding could be applied for authentication of Iceland Moss materials.

KW - Authentication

KW - Cetraria ericetorum

KW - Cetraria islandica

KW - Chemical profiling

KW - DNA barcoding

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DO - 10.1016/j.foodchem.2017.11.073

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SN - 0308-8146

VL - 245

SP - 989

EP - 996

JO - Food Chemistry

JF - Food Chemistry

ER -

Authentication of Iceland Moss (Cetraria islandica) by UPLC-QToF-MS metabolite profiling and DNA barcoding.

Abstract

Bibliographical note

Other keywords

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