Analysis of the fragile histidine triad (FHIT) gene in lobular breast cancer

C. Huiping, J. G. Jonasson, B. A. Agnarsson, B. I. Sigbjornsdottir, K. Huebner, S. Ingvarsson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The fragile histidine triad (FHIT) gene is a candidate tumour suppressor gene in breast and other cancers. We investigated deletions within the FHIT gene in lobular breast cancer and found that 16% of cases showed loss of heterozygosity (LOH) within the gene. We compared LOH within FHIT in lobular and ductal breast tumours and found a significant association between LOH at FHIT and the ductal histological type (P < 0.001). To determine whether genomic alteration of the FHIT gene in lobular breast cancer leads to Fhit inactivation we have assessed the level of Fhit expression by immunohistochemical detection and determined that 27% (15 of 55) consecutive sporadic lobular tumours showed negative or reduced Fhit expression. A significant association was found between LOH at the FHIT gene and reduced Fhit expression in lobular and ductal tumours (P=0.025 and P=0.001, respectively). Thus, genetic alterations within the FHIT gene, leading to loss of Fhit protein, may play an important role in the carcinogenesis of a significant number of sporadic lobular breast cancers, even though the apparent frequency of genomic alterations within the gene is lower than in ductal breast cancer. (C) Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)1552-1557
Number of pages6
JournalEuropean Journal of Cancer
Volume36
Issue number12
DOIs
Publication statusPublished - Aug 2000

Bibliographical note

Funding Information:
The authors thank Sigrun Kristjansdottir and Kristrun Olafsdottir at the Department of Pathology, University Hospital of Iceland, for the immunohistochemical staining. This work was financially supported by the Icelandic Research Council and the Icelandic Cancer Society.

Other keywords

  • FHIT
  • Fhit expression
  • Lobular breast cancer
  • LOH

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