An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study

Stine M. Ulven; Kirsten B. Holven; Amanda Rundblad; Mari C. W. Myhrstad; Lena Leder; Ingrid Dahlman; Vanessa D. de Mello; Ursula Schwab; Carsten Carlberg; Jussi Pihlajamäki; Kjeld Hermansen; Lars O. Dragsted; Ingibjorg Gunnarsdottir; Lieselotte Cloetens; Björn Åkesson; Fredrik Rosqvist; Janne Hukkanen; Karl-Heinz Herzig; Markku J Savolainen; Ulf Risérus; Inga Thorsdottir; Kaisa S Poutanen; Peter Arner; Matti Uusitupa; Marjukka Kolehmainen; Ingibjörg Gunnarsdottir

doi:10.3390/nu11122932

An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study

Stine M. Ulven, Kirsten B. Holven, Amanda Rundblad, Mari C. W. Myhrstad, Lena Leder, Ingrid Dahlman, Vanessa D. de Mello, Ursula Schwab, Carsten Carlberg, Jussi Pihlajamäki, Kjeld Hermansen, Lars O. Dragsted, Ingibjorg Gunnarsdottir, Lieselotte Cloetens, Björn Åkesson, Fredrik Rosqvist, Janne Hukkanen, Karl-Heinz Herzig, Markku J Savolainen, Ulf RisérusInga Thorsdottir, Kaisa S Poutanen, Peter Arner, Matti Uusitupa, Marjukka Kolehmainen, Ingibjörg Gunnarsdottir

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2 Citations (Scopus)

Abstract

A healthy dietary pattern is associated with a lower risk of metabolic syndrome (MetS) and reduced inflammation. To explore this at the molecular level, we investigated the effect of a Nordic diet (ND) on changes in the gene expression profiles of inflammatory and lipid-related genes in peripheral blood mononuclear cells (PBMCs) of individuals with MetS. We hypothesized that the intake of an ND compared to a control diet (CD) would alter the expression of inflammatory genes and genes involved in lipid metabolism. The individuals with MetS underwent an 18/24-week randomized intervention to compare a ND with a CD. Eighty-eight participants (66% women) were included in this sub-study of the larger SYSDIET study. Fasting PBMCs were collected before and after the intervention and changes in gene expression levels were measured using TaqMan Array Micro Fluidic Cards. Forty-eight pre-determined inflammatory and lipid related gene transcripts were analyzed. The expression level of the gene tumor necrosis factor (TNF) receptor superfamily member 1A (TNFRSF1A) was down-regulated (p = 0.004), whereas the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) subunit, RELA proto-oncogene, was up-regulated (p = 0.016) in the ND group compared to the CD group. In conclusion, intake of an ND in individuals with the MetS may affect immune function.

Original language	English
Number of pages	2932
Journal	Nutrients
Volume	11
Issue number	12
DOIs	https://doi.org/10.3390/nu11122932
Publication status	Published - 3 Dec 2019

Other keywords

Metabolic syndrome
Randomized controlled dietary intervention
Gene expression
Peripheral blood mononuclear cells
Inflammation
Efnaskiptasjúkdómar
Bólgur
Mataræði
Genarannsóknir
Diet

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10.3390/nu11122932

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Ulven, S. M., Holven, K. B., Rundblad, A., Myhrstad, M. C. W., Leder, L., Dahlman, I., Mello, V. D. D., Schwab, U., Carlberg, C., Pihlajamäki, J., Hermansen, K., Dragsted, L. O., Gunnarsdottir, I., Cloetens, L., Åkesson, B., Rosqvist, F., Hukkanen, J., Herzig, K-H., Savolainen, M. J., ... Gunnarsdottir, I. (2019). An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study. Nutrients, 11(12). https://doi.org/10.3390/nu11122932

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title = "An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study",

abstract = "A healthy dietary pattern is associated with a lower risk of metabolic syndrome (MetS) and reduced inflammation. To explore this at the molecular level, we investigated the effect of a Nordic diet (ND) on changes in the gene expression profiles of inflammatory and lipid-related genes in peripheral blood mononuclear cells (PBMCs) of individuals with MetS. We hypothesized that the intake of an ND compared to a control diet (CD) would alter the expression of inflammatory genes and genes involved in lipid metabolism. The individuals with MetS underwent an 18/24-week randomized intervention to compare a ND with a CD. Eighty-eight participants (66% women) were included in this sub-study of the larger SYSDIET study. Fasting PBMCs were collected before and after the intervention and changes in gene expression levels were measured using TaqMan Array Micro Fluidic Cards. Forty-eight pre-determined inflammatory and lipid related gene transcripts were analyzed. The expression level of the gene tumor necrosis factor (TNF) receptor superfamily member 1A (TNFRSF1A) was down-regulated (p = 0.004), whereas the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) subunit, RELA proto-oncogene, was up-regulated (p = 0.016) in the ND group compared to the CD group. In conclusion, intake of an ND in individuals with the MetS may affect immune function.",

keywords = "Metabolic syndrome, Randomized controlled dietary intervention, Gene expression, Peripheral blood mononuclear cells, Inflammation, Efnaskiptasj{\'u}kd{\'o}mar, B{\'o}lgur, Matar{\ae}{\dh}i, Genaranns{\'o}knir, Diet, Metabolic syndrome, Randomized controlled dietary intervention, Gene expression, Peripheral blood mononuclear cells, Inflammation, Efnaskiptasj{\'u}kd{\'o}mar, B{\'o}lgur, Matar{\ae}{\dh}i, Genaranns{\'o}knir, Diet",

author = "Ulven, {Stine M.} and Holven, {Kirsten B.} and Amanda Rundblad and Myhrstad, {Mari C. W.} and Lena Leder and Ingrid Dahlman and Mello, {Vanessa D. de} and Ursula Schwab and Carsten Carlberg and Jussi Pihlajam{\"a}ki and Kjeld Hermansen and Dragsted, {Lars O.} and Ingibjorg Gunnarsdottir and Lieselotte Cloetens and Bj{\"o}rn {\AA}kesson and Fredrik Rosqvist and Janne Hukkanen and Karl-Heinz Herzig and Savolainen, {Markku J} and Ulf Ris{\'e}rus and Inga Thorsdottir and Poutanen, {Kaisa S} and Peter Arner and Matti Uusitupa and Marjukka Kolehmainen and Ingibj{\"o}rg Gunnarsdottir",

year = "2019",

month = dec,

day = "3",

doi = "10.3390/nu11122932",

language = "English",

volume = "11",

journal = "Nutrients",

issn = "2072-6643",

publisher = "MDPI Multidisciplinary Digital Publishing Institute",

number = "12",

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Ulven, SM, Holven, KB, Rundblad, A, Myhrstad, MCW, Leder, L, Dahlman, I, Mello, VDD, Schwab, U, Carlberg, C, Pihlajamäki, J, Hermansen, K, Dragsted, LO, Gunnarsdottir, I, Cloetens, L, Åkesson, B, Rosqvist, F, Hukkanen, J, Herzig, K-H, Savolainen, MJ, Risérus, U, Thorsdottir, I, Poutanen, KS, Arner, P, Uusitupa, M, Kolehmainen, M & Gunnarsdottir, I 2019, 'An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study', Nutrients, vol. 11, no. 12. https://doi.org/10.3390/nu11122932

TY - JOUR

T1 - An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study

AU - Ulven, Stine M.

AU - Holven, Kirsten B.

AU - Rundblad, Amanda

AU - Myhrstad, Mari C. W.

AU - Leder, Lena

AU - Dahlman, Ingrid

AU - Mello, Vanessa D. de

AU - Schwab, Ursula

AU - Carlberg, Carsten

AU - Pihlajamäki, Jussi

AU - Hermansen, Kjeld

AU - Dragsted, Lars O.

AU - Gunnarsdottir, Ingibjorg

AU - Cloetens, Lieselotte

AU - Åkesson, Björn

AU - Rosqvist, Fredrik

AU - Hukkanen, Janne

AU - Herzig, Karl-Heinz

AU - Savolainen, Markku J

AU - Risérus, Ulf

AU - Thorsdottir, Inga

AU - Poutanen, Kaisa S

AU - Arner, Peter

AU - Uusitupa, Matti

AU - Kolehmainen, Marjukka

AU - Gunnarsdottir, Ingibjörg

PY - 2019/12/3

Y1 - 2019/12/3

N2 - A healthy dietary pattern is associated with a lower risk of metabolic syndrome (MetS) and reduced inflammation. To explore this at the molecular level, we investigated the effect of a Nordic diet (ND) on changes in the gene expression profiles of inflammatory and lipid-related genes in peripheral blood mononuclear cells (PBMCs) of individuals with MetS. We hypothesized that the intake of an ND compared to a control diet (CD) would alter the expression of inflammatory genes and genes involved in lipid metabolism. The individuals with MetS underwent an 18/24-week randomized intervention to compare a ND with a CD. Eighty-eight participants (66% women) were included in this sub-study of the larger SYSDIET study. Fasting PBMCs were collected before and after the intervention and changes in gene expression levels were measured using TaqMan Array Micro Fluidic Cards. Forty-eight pre-determined inflammatory and lipid related gene transcripts were analyzed. The expression level of the gene tumor necrosis factor (TNF) receptor superfamily member 1A (TNFRSF1A) was down-regulated (p = 0.004), whereas the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) subunit, RELA proto-oncogene, was up-regulated (p = 0.016) in the ND group compared to the CD group. In conclusion, intake of an ND in individuals with the MetS may affect immune function.

AB - A healthy dietary pattern is associated with a lower risk of metabolic syndrome (MetS) and reduced inflammation. To explore this at the molecular level, we investigated the effect of a Nordic diet (ND) on changes in the gene expression profiles of inflammatory and lipid-related genes in peripheral blood mononuclear cells (PBMCs) of individuals with MetS. We hypothesized that the intake of an ND compared to a control diet (CD) would alter the expression of inflammatory genes and genes involved in lipid metabolism. The individuals with MetS underwent an 18/24-week randomized intervention to compare a ND with a CD. Eighty-eight participants (66% women) were included in this sub-study of the larger SYSDIET study. Fasting PBMCs were collected before and after the intervention and changes in gene expression levels were measured using TaqMan Array Micro Fluidic Cards. Forty-eight pre-determined inflammatory and lipid related gene transcripts were analyzed. The expression level of the gene tumor necrosis factor (TNF) receptor superfamily member 1A (TNFRSF1A) was down-regulated (p = 0.004), whereas the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) subunit, RELA proto-oncogene, was up-regulated (p = 0.016) in the ND group compared to the CD group. In conclusion, intake of an ND in individuals with the MetS may affect immune function.

KW - Metabolic syndrome

KW - Randomized controlled dietary intervention

KW - Gene expression

KW - Peripheral blood mononuclear cells

KW - Inflammation

KW - Efnaskiptasjúkdómar

KW - Bólgur

KW - Mataræði

KW - Genarannsóknir

KW - Diet

KW - Metabolic syndrome

KW - Randomized controlled dietary intervention

KW - Gene expression

KW - Peripheral blood mononuclear cells

KW - Inflammation

KW - Efnaskiptasjúkdómar

KW - Bólgur

KW - Mataræði

KW - Genarannsóknir

KW - Diet

U2 - 10.3390/nu11122932

DO - 10.3390/nu11122932

M3 - Article

C2 - 31816875

SN - 2072-6643

VL - 11

JO - Nutrients

JF - Nutrients

IS - 12

ER -

An Isocaloric Nordic Diet Modulates RELA and TNFRSF1A Gene Expression in Peripheral Blood Mononuclear Cells in Individuals with Metabolic Syndrome—A SYSDIET Sub-Study

Abstract

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