An in silico re-design of the metabolism in Thermotoga maritima for increased biohydrogen production

Juan Nogales, Steinn Gudmundsson, Ines Thiele*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Microbial hydrogen production is currently hampered by lack of efficiency. We examine how hydrogen production in the hyperthermophilic bacterium Thermotoga maritima can be increased in silico. An updated genome-scale metabolic model of T. maritima was used to i) describe in detail the H 2 metabolism in this bacterium, ii) identify suitable carbon sources for enhancing H 2 production, and iii) to design knockout strains, which increased the in silico hydrogen production up to 20%. A novel synthetic oxidative module was further designed, which connects the cellular NADPH and ferredoxin pools by inserting into the model a NADPH-ferredoxin reductase. We then combined this in silico knock-in strain with a knockout strain design, resulting in an in silico production strain with a predicted 125% increase in hydrogen yield. The in silico strains designs presented here may serve as blueprints for future metabolic engineering efforts of T. maritima.

Original languageEnglish
Pages (from-to)12205-12218
Number of pages14
JournalInternational Journal of Hydrogen Energy
Issue number17
Publication statusPublished - Sep 2012

Bibliographical note

Funding Information:
This work was supported by the U.S. Department of Energy , Offices of Advanced Scientific Computing Research and the Biological and Environmental Research as part of the Scientific Discovery Through Advanced Computing program, grant DE-SC0002009 . JN was funded, in part, by the Spanish MEC through National Plan of I-D+i 2008–2011. The authors thank Dr. Ronan M.T. Fleming for valuable discussions.

Other keywords

  • Biohydrogen
  • Biosustainability
  • COBRA methods
  • Genome-scale model
  • Thermotoga maritima


Dive into the research topics of 'An in silico re-design of the metabolism in Thermotoga maritima for increased biohydrogen production'. Together they form a unique fingerprint.

Cite this