Abstract
Silica, precipitated out of geothermal fluid discharged from a geothermal powerplant in Svartsengi on the Reykjanes peninsula in Iceland, was used as a chromatographic adsorbent to extract blue colored protein, C-phycocyanin, from coccoid blue-green algae. The only supplement used was salt obtained by evaporating the geothermal fluid. Analysis of the silica, using scanning electron microscopy, X-ray diffractometry and Brunauer-Emmett-Teller (BET) adsorption confirmed it has a high specific surface area and is amorphous. Upon adsorption and subsequent elution the purity of the extracted protein, measured as the ratio of the light absorbance of 620 and 280. nm, increased from 0.5 to above 2.0. Our results could facilitate utilization of a mostly unused byproduct of geothermal powerplants as chromatographic material.
Original language | English |
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Pages (from-to) | 30-34 |
Number of pages | 5 |
Journal | Geothermics |
Volume | 50 |
DOIs | |
Publication status | Published - Apr 2014 |
Bibliographical note
Funding Information:This research was supported in part by the Icelandic Technology Development Fund and the Energy Fund of the National Energy Authority .
Other keywords
- Adsorption
- Chromatographic
- Geothermal silica
- Phycocyanin
- Protein separation