A stress protein is induced in the deep-sea barophilic hyperthermophile Thermococcus barophilus when grown under atmospheric pressure

Viggó Thór Marteinsson*, Anna Louise Reysenbach, Jean Louis Birrien, Daniel Prieur

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The whole-cell protein inventory of the deep-sea barophilic hyperthermophile Thermococcus barophilus was examined by one-dimensional SDS gradient gel electrophoresis when grown under different pressure conditions at 85°C (T(opt)). One protein (P60) with a molecular mass of approximately 60 kDa was prominent at low pressures (0.3 MPa hydrostatic pressure and 0.1 MPa atmospheric pressure) but not at deep-sea pressures (10, 30, and 40 MPa). About 17 amino acids were sequenced from the N-terminal end of the protein. Sequence homology analysis in the GenBank database showed that P60 most closely resembled heat-shock proteins in some sulfur-metabolizing Archaea. A high degree of amino acid identity (81%-93%) to thermosome subunits in Thermococcales strains was found. Another protein (P35) with molecular mass of approximately 35.5 kDa was induced at 40 MPa hydrostatic pressure but not under low-pressure conditions. No amino acid sequence homology was found for this protein when the 40 amino acids from the N-terminal end were compared with homologous regions of proteins from databases. A PTk diagram was generated for T. barophilus. The results suggest that P(habitat) is about 35 MPa, which corresponds to the in situ pressure where the strain was obtained.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalExtremophiles
Volume3
Issue number4
DOIs
Publication statusPublished - Nov 1999

Bibliographical note

Funding Information:
Acknowledgments We thank M. Vernet for technical assistance in pressure experiments and P. Potin and L. Vogel for discussions. V. Marteinsson was supported by a French-Icelandic fellowship. This work was supported by GDR, CNRS/IFREMER, the CPER 94-95, European Union (Feder) and Conseil Général du Finistère.

Other keywords

  • Barophile
  • Heat-shock protein
  • Hyperthermophile
  • Pressure
  • Thermococcus barophilus

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