A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods

R. E. Hartnell; L. Stockley; W. Keay; J. P. Rosec; D. Hervio-Heath; H. Van den Berg; F. Leoni; D. Ottaviani; U. Henigman; S. Denayer; B. Serbruyns; F. Georgsson; G. Krumova-Valcheva; E. Gyurova; C. Blanco; S. Copin; E. Strauch; K. Wieczorek; M. Lopatek; A. Britova; G. Hardouin; B. Lombard; P. in't Veld; A. Leclercq; C. Baker-Austin

doi:10.1016/j.ijfoodmicro.2018.02.008

A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods

R. E. Hartnell, L. Stockley, W. Keay, J. P. Rosec, D. Hervio-Heath, H. Van den Berg, F. Leoni, D. Ottaviani, U. Henigman, S. Denayer, B. Serbruyns, F. Georgsson, G. Krumova-Valcheva, E. Gyurova, C. Blanco, S. Copin, E. Strauch, K. Wieczorek, M. Lopatek, A. BritovaG. Hardouin, B. Lombard, P. in't Veld, A. Leclercq, C. Baker-Austin^*

^*Corresponding author for this work

Faculty of Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also considered simplification of the biochemical confirmation steps. In addition, because of advances in molecular based methods for identification of human pathogenic Vibrio spp. classical and real-time PCR options were also included within the scope of the validation. These considerations formed the basis of a multi-laboratory validation study with the aim of improving the precision of this ISO technical specification and providing a single ISO standard method to enable detection of these important foodborne Vibrio spp. To achieve this aim, an international validation study involving 13 laboratories from 9 countries in Europe was conducted in 2013. The results of this validation have enabled integration of the two existing technical specifications targeting the detection of the major foodborne Vibrio spp., simplification of the suite of recommended biochemical identification tests and the introduction of molecular procedures that provide both species level identification and discrimination of putatively pathogenic strains of V. parahaemolyticus by the determination of the presence of theromostable direct and direct related haemolysins. The method performance characteristics generated in this have been included in revised international standard, ISO 21872:2017, published in July 2017.

Original language	English
Pages (from-to)	58-65
Number of pages	8
Journal	International Journal of Food Microbiology
Volume	288
DOIs	https://doi.org/10.1016/j.ijfoodmicro.2018.02.008
Publication status	Published - 2 Jan 2019

Bibliographical note

Funding Information:
The validation of International Standard 21872 was carried out under the framework of European Mandate No. ISO 21872 of DG SANTE and DG GROW (European Commission). Authors wish to thank all participants in the collaborative trials from the following institutes: European Union Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Molluscs, Centre for Fisheries, Environment and Aquaculture Science, Barrack Road, The Nothe, Weymouth, United Kingdom, DT4 8UB. Service Commun des Laboratoires Minist?re de l? Economie et des Finances, Montpellier, France. LNR Microbiologie des Coquillages, Centre Ifremer de Brest, Laboratoire Microbiologie (EMP/MIC), BP 70, 29280 Plouzane, France. National Institute of Public Health and the Environment (RIVM), PO Box 1, Antonie van Leeuwenhoeklaan 9, 3720 BA Bilthoven The Netherlands. Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Sezione di Ancona, Italy. Institute for food hygiene and bromatology, Veterinary faculty, Gerbiceva 60, SI ? 1000, Ljubljana, Slovenia. Scientific Institute of Public Health, Scientific Service of Food borne Pathogens, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium. Chemiphar n.v, Lieven Bauwensstraat 4, 8200 Brugge, Belgium. Icelandic Food and Biotech R&D, Matvaelarannsoknir Islands, Skulagotu 4, Icelandic Food and Biotech R&D V?nlandslei? 12, 113 Reykjav?k, Iceland, 15 Pencho Slaveikov blvd., Sofia 1606, Bulgaria. Agencia Espa?ola de Sguridad Alimentaria. Centro Nacional de Alimentaci?n. Servicio de Microbiolog?a Alimentaria Carretera de Majadahonda a Pozuelo. Km, 5,2 Majadahonda, 28220 Madrid, Spain. ANSES, Laboratoire des produits de la p?che de Boulogne-sur-mer, Boulevard du Bassin Napol?on, 62200 Boulogne sur Mer, France. Bundesinstitut f?r Risikobewertung (BfR), German Federal Institute for Risk Assessment, Berlin, Germany. Dept of Food & Environmental Virology to Department of Hygiene of Food of Animal Origin. State Veterinary and Food Institute, Janoskova 1611/58 026 01 Dolny Kubin, Slovakia.

Funding Information:
The validation of International Standard 21872 was carried out under the framework of European Mandate No. ISO 21872 of DG SANTE and DG GROW (European Commission). Authors wish to thank all participants in the collaborative trials from the following institutes: European Union Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Molluscs, Centre for Fisheries, Environment and Aquaculture Science, Barrack Road, The Nothe, Weymouth, United Kingdom, DT4 8UB. Service Commun des Laboratoires Ministère de l’ Economie et des Finances, Montpellier, France. LNR Microbiologie des Coquillages, Centre Ifremer de Brest, Laboratoire Microbiologie (EMP/MIC), BP 70, 29280 Plouzane, France. National Institute of Public Health and the Environment (RIVM), PO Box 1, Antonie van Leeuwenhoeklaan 9, 3720 BA Bilthoven The Netherlands. Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Sezione di Ancona, Italy. Institute for food hygiene and bromatology, Veterinary faculty, Gerbiceva 60, SI – 1000, Ljubljana, Slovenia. Scientific Institute of Public Health, Scientific Service of Food borne Pathogens, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium. Chemiphar n.v, Lieven Bauwensstraat 4, 8200 Brugge, Belgium. Icelandic Food and Biotech R&D, Matvaelarannsoknir Islands, Skulagotu 4, Icelandic Food and Biotech R&D Vínlandsleið 12, 113 Reykjavík, Iceland, 15 Pencho Slaveikov blvd., Sofia 1606, Bulgaria. Agencia Española de Sguridad Alimentaria. Centro Nacional de Alimentación. Servicio de Microbiología Alimentaria Carretera de Majadahonda a Pozuelo. Km, 5,2 Majadahonda, 28220 Madrid, Spain. ANSES, Laboratoire des produits de la pêche de Boulogne-sur-mer, Boulevard du Bassin Napoléon, 62200 Boulogne sur Mer, France. Bundesinstitut für Risikobewertung (BfR), German Federal Institute for Risk Assessment, Berlin, Germany. Dept of Food & Environmental Virology to Department of Hygiene of Food of Animal Origin. State Veterinary and Food Institute, Janoskova 1611/58 026 01 Dolny Kubin, Slovakia.

Publisher Copyright:
© 2018

Other keywords

Biochemical methods
Conventional PCR
Oysters
Prawns
Real-time PCR
Seafood
Vibrios

Access to Document

10.1016/j.ijfoodmicro.2018.02.008

Cite this

Hartnell, R. E., Stockley, L., Keay, W., Rosec, J. P., Hervio-Heath, D., Van den Berg, H., Leoni, F., Ottaviani, D., Henigman, U., Denayer, S., Serbruyns, B., Georgsson, F., Krumova-Valcheva, G., Gyurova, E., Blanco, C., Copin, S., Strauch, E., Wieczorek, K., Lopatek, M., ... Baker-Austin, C. (2019). A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods. International Journal of Food Microbiology, 288, 58-65. https://doi.org/10.1016/j.ijfoodmicro.2018.02.008

@article{e3ade581348147de86d92de93d329d84,

title = "A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods",

abstract = "Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also considered simplification of the biochemical confirmation steps. In addition, because of advances in molecular based methods for identification of human pathogenic Vibrio spp. classical and real-time PCR options were also included within the scope of the validation. These considerations formed the basis of a multi-laboratory validation study with the aim of improving the precision of this ISO technical specification and providing a single ISO standard method to enable detection of these important foodborne Vibrio spp. To achieve this aim, an international validation study involving 13 laboratories from 9 countries in Europe was conducted in 2013. The results of this validation have enabled integration of the two existing technical specifications targeting the detection of the major foodborne Vibrio spp., simplification of the suite of recommended biochemical identification tests and the introduction of molecular procedures that provide both species level identification and discrimination of putatively pathogenic strains of V. parahaemolyticus by the determination of the presence of theromostable direct and direct related haemolysins. The method performance characteristics generated in this have been included in revised international standard, ISO 21872:2017, published in July 2017.",

keywords = "Biochemical methods, Conventional PCR, Oysters, Prawns, Real-time PCR, Seafood, Vibrios",

author = "Hartnell, {R. E.} and L. Stockley and W. Keay and Rosec, {J. P.} and D. Hervio-Heath and {Van den Berg}, H. and F. Leoni and D. Ottaviani and U. Henigman and S. Denayer and B. Serbruyns and F. Georgsson and G. Krumova-Valcheva and E. Gyurova and C. Blanco and S. Copin and E. Strauch and K. Wieczorek and M. Lopatek and A. Britova and G. Hardouin and B. Lombard and {in't Veld}, P. and A. Leclercq and C. Baker-Austin",

note = "Funding Information: The validation of International Standard 21872 was carried out under the framework of European Mandate No. ISO 21872 of DG SANTE and DG GROW (European Commission). Authors wish to thank all participants in the collaborative trials from the following institutes: European Union Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Molluscs, Centre for Fisheries, Environment and Aquaculture Science, Barrack Road, The Nothe, Weymouth, United Kingdom, DT4 8UB. Service Commun des Laboratoires Minist?re de l? Economie et des Finances, Montpellier, France. LNR Microbiologie des Coquillages, Centre Ifremer de Brest, Laboratoire Microbiologie (EMP/MIC), BP 70, 29280 Plouzane, France. National Institute of Public Health and the Environment (RIVM), PO Box 1, Antonie van Leeuwenhoeklaan 9, 3720 BA Bilthoven The Netherlands. Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Sezione di Ancona, Italy. Institute for food hygiene and bromatology, Veterinary faculty, Gerbiceva 60, SI ? 1000, Ljubljana, Slovenia. Scientific Institute of Public Health, Scientific Service of Food borne Pathogens, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium. Chemiphar n.v, Lieven Bauwensstraat 4, 8200 Brugge, Belgium. Icelandic Food and Biotech R&D, Matvaelarannsoknir Islands, Skulagotu 4, Icelandic Food and Biotech R&D V?nlandslei? 12, 113 Reykjav?k, Iceland, 15 Pencho Slaveikov blvd., Sofia 1606, Bulgaria. Agencia Espa?ola de Sguridad Alimentaria. Centro Nacional de Alimentaci?n. Servicio de Microbiolog?a Alimentaria Carretera de Majadahonda a Pozuelo. Km, 5,2 Majadahonda, 28220 Madrid, Spain. ANSES, Laboratoire des produits de la p?che de Boulogne-sur-mer, Boulevard du Bassin Napol?on, 62200 Boulogne sur Mer, France. Bundesinstitut f?r Risikobewertung (BfR), German Federal Institute for Risk Assessment, Berlin, Germany. Dept of Food & Environmental Virology to Department of Hygiene of Food of Animal Origin. State Veterinary and Food Institute, Janoskova 1611/58 026 01 Dolny Kubin, Slovakia. Funding Information: The validation of International Standard 21872 was carried out under the framework of European Mandate No. ISO 21872 of DG SANTE and DG GROW (European Commission). Authors wish to thank all participants in the collaborative trials from the following institutes: European Union Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Molluscs, Centre for Fisheries, Environment and Aquaculture Science, Barrack Road, The Nothe, Weymouth, United Kingdom, DT4 8UB. Service Commun des Laboratoires Minist{\`e}re de l{\textquoteright} Economie et des Finances, Montpellier, France. LNR Microbiologie des Coquillages, Centre Ifremer de Brest, Laboratoire Microbiologie (EMP/MIC), BP 70, 29280 Plouzane, France. National Institute of Public Health and the Environment (RIVM), PO Box 1, Antonie van Leeuwenhoeklaan 9, 3720 BA Bilthoven The Netherlands. Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Sezione di Ancona, Italy. Institute for food hygiene and bromatology, Veterinary faculty, Gerbiceva 60, SI – 1000, Ljubljana, Slovenia. Scientific Institute of Public Health, Scientific Service of Food borne Pathogens, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium. Chemiphar n.v, Lieven Bauwensstraat 4, 8200 Brugge, Belgium. Icelandic Food and Biotech R&D, Matvaelarannsoknir Islands, Skulagotu 4, Icelandic Food and Biotech R&D V{\'i}nlandslei{\dh} 12, 113 Reykjav{\'i}k, Iceland, 15 Pencho Slaveikov blvd., Sofia 1606, Bulgaria. Agencia Espa{\~n}ola de Sguridad Alimentaria. Centro Nacional de Alimentaci{\'o}n. Servicio de Microbiolog{\'i}a Alimentaria Carretera de Majadahonda a Pozuelo. Km, 5,2 Majadahonda, 28220 Madrid, Spain. ANSES, Laboratoire des produits de la p{\^e}che de Boulogne-sur-mer, Boulevard du Bassin Napol{\'e}on, 62200 Boulogne sur Mer, France. Bundesinstitut f{\"u}r Risikobewertung (BfR), German Federal Institute for Risk Assessment, Berlin, Germany. Dept of Food & Environmental Virology to Department of Hygiene of Food of Animal Origin. State Veterinary and Food Institute, Janoskova 1611/58 026 01 Dolny Kubin, Slovakia. Publisher Copyright: {\textcopyright} 2018",

year = "2019",

month = jan,

day = "2",

doi = "10.1016/j.ijfoodmicro.2018.02.008",

language = "English",

volume = "288",

pages = "58--65",

journal = "International Journal of Food Microbiology",

issn = "0168-1605",

publisher = "Elsevier B.V.",

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Hartnell, RE, Stockley, L, Keay, W, Rosec, JP, Hervio-Heath, D, Van den Berg, H, Leoni, F, Ottaviani, D, Henigman, U, Denayer, S, Serbruyns, B, Georgsson, F, Krumova-Valcheva, G, Gyurova, E, Blanco, C, Copin, S, Strauch, E, Wieczorek, K, Lopatek, M, Britova, A, Hardouin, G, Lombard, B, in't Veld, P, Leclercq, A & Baker-Austin, C 2019, 'A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods', International Journal of Food Microbiology, vol. 288, pp. 58-65. https://doi.org/10.1016/j.ijfoodmicro.2018.02.008

TY - JOUR

T1 - A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods

AU - Hartnell, R. E.

AU - Stockley, L.

AU - Keay, W.

AU - Rosec, J. P.

AU - Hervio-Heath, D.

AU - Van den Berg, H.

AU - Leoni, F.

AU - Ottaviani, D.

AU - Henigman, U.

AU - Denayer, S.

AU - Serbruyns, B.

AU - Georgsson, F.

AU - Krumova-Valcheva, G.

AU - Gyurova, E.

AU - Blanco, C.

AU - Copin, S.

AU - Strauch, E.

AU - Wieczorek, K.

AU - Lopatek, M.

AU - Britova, A.

AU - Hardouin, G.

AU - Lombard, B.

AU - in't Veld, P.

AU - Leclercq, A.

AU - Baker-Austin, C.

N1 - Funding Information: The validation of International Standard 21872 was carried out under the framework of European Mandate No. ISO 21872 of DG SANTE and DG GROW (European Commission). Authors wish to thank all participants in the collaborative trials from the following institutes: European Union Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Molluscs, Centre for Fisheries, Environment and Aquaculture Science, Barrack Road, The Nothe, Weymouth, United Kingdom, DT4 8UB. Service Commun des Laboratoires Minist?re de l? Economie et des Finances, Montpellier, France. LNR Microbiologie des Coquillages, Centre Ifremer de Brest, Laboratoire Microbiologie (EMP/MIC), BP 70, 29280 Plouzane, France. National Institute of Public Health and the Environment (RIVM), PO Box 1, Antonie van Leeuwenhoeklaan 9, 3720 BA Bilthoven The Netherlands. Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Sezione di Ancona, Italy. Institute for food hygiene and bromatology, Veterinary faculty, Gerbiceva 60, SI ? 1000, Ljubljana, Slovenia. Scientific Institute of Public Health, Scientific Service of Food borne Pathogens, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium. Chemiphar n.v, Lieven Bauwensstraat 4, 8200 Brugge, Belgium. Icelandic Food and Biotech R&D, Matvaelarannsoknir Islands, Skulagotu 4, Icelandic Food and Biotech R&D V?nlandslei? 12, 113 Reykjav?k, Iceland, 15 Pencho Slaveikov blvd., Sofia 1606, Bulgaria. Agencia Espa?ola de Sguridad Alimentaria. Centro Nacional de Alimentaci?n. Servicio de Microbiolog?a Alimentaria Carretera de Majadahonda a Pozuelo. Km, 5,2 Majadahonda, 28220 Madrid, Spain. ANSES, Laboratoire des produits de la p?che de Boulogne-sur-mer, Boulevard du Bassin Napol?on, 62200 Boulogne sur Mer, France. Bundesinstitut f?r Risikobewertung (BfR), German Federal Institute for Risk Assessment, Berlin, Germany. Dept of Food & Environmental Virology to Department of Hygiene of Food of Animal Origin. State Veterinary and Food Institute, Janoskova 1611/58 026 01 Dolny Kubin, Slovakia. Funding Information: The validation of International Standard 21872 was carried out under the framework of European Mandate No. ISO 21872 of DG SANTE and DG GROW (European Commission). Authors wish to thank all participants in the collaborative trials from the following institutes: European Union Reference Laboratory for Monitoring Bacteriological and Viral Contamination of Bivalve Molluscs, Centre for Fisheries, Environment and Aquaculture Science, Barrack Road, The Nothe, Weymouth, United Kingdom, DT4 8UB. Service Commun des Laboratoires Ministère de l’ Economie et des Finances, Montpellier, France. LNR Microbiologie des Coquillages, Centre Ifremer de Brest, Laboratoire Microbiologie (EMP/MIC), BP 70, 29280 Plouzane, France. National Institute of Public Health and the Environment (RIVM), PO Box 1, Antonie van Leeuwenhoeklaan 9, 3720 BA Bilthoven The Netherlands. Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Sezione di Ancona, Italy. Institute for food hygiene and bromatology, Veterinary faculty, Gerbiceva 60, SI – 1000, Ljubljana, Slovenia. Scientific Institute of Public Health, Scientific Service of Food borne Pathogens, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium. Chemiphar n.v, Lieven Bauwensstraat 4, 8200 Brugge, Belgium. Icelandic Food and Biotech R&D, Matvaelarannsoknir Islands, Skulagotu 4, Icelandic Food and Biotech R&D Vínlandsleið 12, 113 Reykjavík, Iceland, 15 Pencho Slaveikov blvd., Sofia 1606, Bulgaria. Agencia Española de Sguridad Alimentaria. Centro Nacional de Alimentación. Servicio de Microbiología Alimentaria Carretera de Majadahonda a Pozuelo. Km, 5,2 Majadahonda, 28220 Madrid, Spain. ANSES, Laboratoire des produits de la pêche de Boulogne-sur-mer, Boulevard du Bassin Napoléon, 62200 Boulogne sur Mer, France. Bundesinstitut für Risikobewertung (BfR), German Federal Institute for Risk Assessment, Berlin, Germany. Dept of Food & Environmental Virology to Department of Hygiene of Food of Animal Origin. State Veterinary and Food Institute, Janoskova 1611/58 026 01 Dolny Kubin, Slovakia. Publisher Copyright: © 2018

PY - 2019/1/2

Y1 - 2019/1/2

N2 - Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also considered simplification of the biochemical confirmation steps. In addition, because of advances in molecular based methods for identification of human pathogenic Vibrio spp. classical and real-time PCR options were also included within the scope of the validation. These considerations formed the basis of a multi-laboratory validation study with the aim of improving the precision of this ISO technical specification and providing a single ISO standard method to enable detection of these important foodborne Vibrio spp. To achieve this aim, an international validation study involving 13 laboratories from 9 countries in Europe was conducted in 2013. The results of this validation have enabled integration of the two existing technical specifications targeting the detection of the major foodborne Vibrio spp., simplification of the suite of recommended biochemical identification tests and the introduction of molecular procedures that provide both species level identification and discrimination of putatively pathogenic strains of V. parahaemolyticus by the determination of the presence of theromostable direct and direct related haemolysins. The method performance characteristics generated in this have been included in revised international standard, ISO 21872:2017, published in July 2017.

AB - Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872–1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872–2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also considered simplification of the biochemical confirmation steps. In addition, because of advances in molecular based methods for identification of human pathogenic Vibrio spp. classical and real-time PCR options were also included within the scope of the validation. These considerations formed the basis of a multi-laboratory validation study with the aim of improving the precision of this ISO technical specification and providing a single ISO standard method to enable detection of these important foodborne Vibrio spp. To achieve this aim, an international validation study involving 13 laboratories from 9 countries in Europe was conducted in 2013. The results of this validation have enabled integration of the two existing technical specifications targeting the detection of the major foodborne Vibrio spp., simplification of the suite of recommended biochemical identification tests and the introduction of molecular procedures that provide both species level identification and discrimination of putatively pathogenic strains of V. parahaemolyticus by the determination of the presence of theromostable direct and direct related haemolysins. The method performance characteristics generated in this have been included in revised international standard, ISO 21872:2017, published in July 2017.

KW - Biochemical methods

KW - Conventional PCR

KW - Oysters

KW - Prawns

KW - Real-time PCR

KW - Seafood

KW - Vibrios

UR - http://www.scopus.com/inward/record.url?scp=85044122342&partnerID=8YFLogxK

U2 - 10.1016/j.ijfoodmicro.2018.02.008

DO - 10.1016/j.ijfoodmicro.2018.02.008

M3 - Article

C2 - 29571579

AN - SCOPUS:85044122342

SN - 0168-1605

VL - 288

SP - 58

EP - 65

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

ER -

A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods

Abstract

Bibliographical note

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