11ß-hydroxysteroid dehydrogenase in the ovary: High expression in the oocyte.: High expression in the oocyte

Rafn Benediktsson; J. L.W. Yau; S. Low; L. P. Brett; B. E. Cooke; C. R.W. Edwards; Jonathan R. Seckl

doi:10.1677/joe.0.1350053

11ß-hydroxysteroid dehydrogenase in the ovary: High expression in the oocyte. High expression in the oocyte

Rafn Benediktsson, J. L.W. Yau, S. Low , L. P. Brett, B. E. Cooke, C. R.W. Edwards, Jonathan R. Seckl

Research output: Contribution to journal › Article › peer-review

Abstract

The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) catalyses the conversion of physiological glucocorticoids to inactive products, thus modifying the access of glucocorticoids to glucocorticoid and mineralocorticoid receptors. Glucocorticoids may affect ovarian function both indirectly and via binding to ovarian receptors. We have demonstrated 11β-HSD bioactivity and mRNA expression in rat ovary in vitro. The enzyme was localized to oocytes and luteal bodies immunohistochemically using two antibodies raised against purified rat liver 11β-HSD. These data are supported by in-situ hybridization studies, which also localized 11β-HSD mRNA expression to oocytes and luteal bodies. The results suggest that 11β-HSD may modulate the effects of glucocorticoid on ovarian function.

Original language	English
Pages (from-to)	53-58
Number of pages	6
Journal	Journal of Endocrinological Investigation
Volume	135
Issue number	1
DOIs	https://doi.org/10.1677/joe.0.1350053
Publication status	Published - 1992

Access to Document

10.1677/joe.0.1350053

Cite this

@article{73e111cb8bf84e15b369403725cfc498,

title = "11{\ss}-hydroxysteroid dehydrogenase in the ovary: High expression in the oocyte.: High expression in the oocyte",

abstract = "The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) catalyses the conversion of physiological glucocorticoids to inactive products, thus modifying the access of glucocorticoids to glucocorticoid and mineralocorticoid receptors. Glucocorticoids may affect ovarian function both indirectly and via binding to ovarian receptors. We have demonstrated 11β-HSD bioactivity and mRNA expression in rat ovary in vitro. The enzyme was localized to oocytes and luteal bodies immunohistochemically using two antibodies raised against purified rat liver 11β-HSD. These data are supported by in-situ hybridization studies, which also localized 11β-HSD mRNA expression to oocytes and luteal bodies. The results suggest that 11β-HSD may modulate the effects of glucocorticoid on ovarian function.",

author = "Rafn Benediktsson and Yau, {J. L.W.} and S. Low and Brett, {L. P.} and Cooke, {B. E.} and Edwards, {C. R.W.} and Seckl, {Jonathan R.}",

year = "1992",

doi = "10.1677/joe.0.1350053",

language = "English",

volume = "135",

pages = "53--58",

journal = "Journal of Endocrinological Investigation",

issn = "0391-4097",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "1",

}

TY - JOUR

T1 - 11ß-hydroxysteroid dehydrogenase in the ovary: High expression in the oocyte.

T2 - High expression in the oocyte

AU - Benediktsson, Rafn

AU - Yau, J. L.W.

AU - Low , S.

AU - Brett, L. P.

AU - Cooke, B. E.

AU - Edwards, C. R.W.

AU - Seckl, Jonathan R.

PY - 1992

Y1 - 1992

N2 - The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) catalyses the conversion of physiological glucocorticoids to inactive products, thus modifying the access of glucocorticoids to glucocorticoid and mineralocorticoid receptors. Glucocorticoids may affect ovarian function both indirectly and via binding to ovarian receptors. We have demonstrated 11β-HSD bioactivity and mRNA expression in rat ovary in vitro. The enzyme was localized to oocytes and luteal bodies immunohistochemically using two antibodies raised against purified rat liver 11β-HSD. These data are supported by in-situ hybridization studies, which also localized 11β-HSD mRNA expression to oocytes and luteal bodies. The results suggest that 11β-HSD may modulate the effects of glucocorticoid on ovarian function.

AB - The enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) catalyses the conversion of physiological glucocorticoids to inactive products, thus modifying the access of glucocorticoids to glucocorticoid and mineralocorticoid receptors. Glucocorticoids may affect ovarian function both indirectly and via binding to ovarian receptors. We have demonstrated 11β-HSD bioactivity and mRNA expression in rat ovary in vitro. The enzyme was localized to oocytes and luteal bodies immunohistochemically using two antibodies raised against purified rat liver 11β-HSD. These data are supported by in-situ hybridization studies, which also localized 11β-HSD mRNA expression to oocytes and luteal bodies. The results suggest that 11β-HSD may modulate the effects of glucocorticoid on ovarian function.

UR - http://www.scopus.com/inward/record.url?scp=0026757114&partnerID=8YFLogxK

U2 - 10.1677/joe.0.1350053

DO - 10.1677/joe.0.1350053

M3 - Article

C2 - 1431683

AN - SCOPUS:0026757114

SN - 0391-4097

VL - 135

SP - 53

EP - 58

JO - Journal of Endocrinological Investigation

JF - Journal of Endocrinological Investigation

IS - 1

ER -

11ß-hydroxysteroid dehydrogenase in the ovary: High expression in the oocyte. High expression in the oocyte

Abstract

Access to Document

Fingerprint

Cite this